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Dietary Thiocyanate and N-Nitrosation in vivo in the Wistar Rat

Maduagwu, E. N. and Umoh, I. B. (1988) Dietary Thiocyanate and N-Nitrosation in vivo in the Wistar Rat. Annal of Nutrition and Metabolism, 32 (1). pp. 20-37. ISSN 0250-6807 (Print) eISSN: 1421-9697 (Online)

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Abstract

The influence of dietary thiocyanate (SCN-) on N-nitrosation in vivo was investigated over 14 min following the administration, by stomach tube, of single doses of sodium nitrite and dimethylamine hydrochloride to male albino Wistar rats whose diet contained appreciable quantities of bound cyanide (cyanogenic glycosides) and free (nonglycosidic) cyanide. The rate of disappearance of the nitrosating agent (NO-2 from the stomach in control animals showed a high linear correlation (r = -0.84) with gastric N-nitrosation, as measured by metabolism (N-demethylation) of ‘formed’ nitrosamine in liver tissue isolated from these animals. This statistical relationship was significantly increased (r = 0.98), as well as the rate of NO-2 utilization and the activity of the N-demethylase enzyme, in animals fed the test diet. Stomach SCN- content was well correlated with both stomach NO-2 concentration (r = -0.95) and liver N-demethylase activity (r = +0.93). Interactions, in vivo, between thiocyanate ion and nitrosamine precursors ingested in food may enhance nitrosamine carcinogenesis.

Item Type: Article
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history > QH301 Biology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 20 Mar 2017 16:06
Last Modified: 20 Mar 2017 16:06
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/7982

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