Pro-inflammatory and toxicological evaluation of Hepacare� in mice

Adebayo, H. A. and Ashano, Efejiro and Yakubu, Omolara Faith and Okubena, O. (2017) Pro-inflammatory and toxicological evaluation of Hepacare� in mice. Journal of Taibah University Medical Sciences, 12 (4). pp. 313-323.

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Abstract

Objectives: Hepacare� is a widely marketed herbal formulation in Nigeria for treating chronic liver ailments. This study evaluated the safety, as well as proinflammatory and genotoxicity effects, of Hepacare� in mice. Methods: The effect of the formulation was estimated in a 28-day study where 25 mice were divided into five groups, and Hepacare� was orally administered at 250, 500, 750 and 2500 mg/kg body weight. The biochemical and haematological parameters were determined, organ weights were estimated and histopathology was also conducted. mRNA expression of the pro-inflammatory cytokines, TNF-a and IL-6 was estimated by RT-PCR in acute toxicity experiments. Results: The LD50 was calculated at 3807.89 mg/kg body weight in mice. There was a significant increase (p < 0.05) in the ALP activity in the 750 mg/kg treated group, while the 2500 mg/kg group exhibited significant increases in their AST, ALT, ALP, total bilirubin and total protein levels compared with the control group. However, there was a significant dose related increase in monocytes

Item Type:	Article
Uncontrolled Keywords:	Genotoxicity; Hepacare; Herbal formulation; Proinflammation; Toxicity studies
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mrs Patricia Nwokealisi
Date Deposited:	29 Aug 2017 13:36
Last Modified:	29 Aug 2017 13:36
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/8972

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