GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF VIBURNUM OPULUS (L) EXTRACT AND ITS TOXICITY STUDIES IN RATS

Adebayo, H. A. and Alade, A. B. and Yakubu, Omolara Faith (2017) GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF VIBURNUM OPULUS (L) EXTRACT AND ITS TOXICITY STUDIES IN RATS. Asian Journal of Pharmaceutical and Clinical Research, 10 (6). pp. 383-388. ISSN 2455-3891,Print - 0974-2441

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Abstract

Objectives: This study was aimed at establishing the antimicrobial and phytochemical profiles of Viburnum opulus (L) as well as the safety potential of the extract in albino Wistar rats. Methods: Ethanol, n-hexane, ethyl acetate, butanol, and water fractions were prepared for both phytochemical assessments using gas chromatographymass spectrometry analysis (GC-MS). Five groups of seven rats were used for the study. Group A received distilled water (control), while Groups B to E were treated, respectively, with 250, 500, 1000, and 1500 mg/kg body weight of V. opulus extract by abdominal canalization for 28 days. Blood samples were obtained for biochemical analyses, and the liver tissues were further processed for histological studies. Results: The GC-MS spectra revealed the existence of various phytoconstituents such as neophytadiene, germaciene, and caryophyllene among others. High-density lipoprotein and albumin were significantly (p<0.05) elevated in animals administered with 500, 1000, and 1500 mg/kg bw of the leaf extract. Ethanol, butanol, and water fractions of the leaf of V. opulus showed antimicrobial action against most of the organisms used in this study. Conclusion: The result indicates that V. opulus leaf extract contains a wild range of fatty acids and heterocyclic compounds with antimicrobial efficacy and showing no hepatic damage.

Item Type:	Article
Uncontrolled Keywords:	Viburnum opulus, Gas chromatography-mass spectrometry analysis, Biochemical, Antimicrobial, Histology
Subjects:	Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mrs Patricia Nwokealisi
Date Deposited:	29 Aug 2017 13:42
Last Modified:	29 Aug 2017 13:42
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/8973

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