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Ex-vivo response of Plasmodium falciparum to Dihydroartemisinin in an area of Ota South-west Nigeria

Dokunmu, Titilope M. and Akinbohun, Adesola E and Rotimi, S. O and Rotimi , Oluwakemi A and Ogunlana, Olubanke O. (2016) Ex-vivo response of Plasmodium falciparum to Dihydroartemisinin in an area of Ota South-west Nigeria. In: Experimental Biology 2016 Meeting, April, USA.

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Artemisinin drugs remain the mainstay of falciparum malaria control globally. Ring-stage survival assay (RSA) is a simple new method that can be used for detecting artemisinin resistance, thus this study aims to determine ex-vivo parasite susceptibility to dihydroartemisinin and its correlation with in-vivo parasite response to artemisinin combinations in an endemic area of Nigeria. Using the simple and sensitive ring-stage survival assay (RSA), 50 parasite isolates from patients with malaria were exposed to DHA concentrations for 6 hours and ring stage survival was determined by microscopy within 72hours of drug exposure. In-vivo parasite clearance was compared with parasite survival in matched patient samples. Parasitemia cleared rapidly within 24hours (mean 22.4 ± 0.2h) in all subjects. There was a dose-dependent growth inhibition in drug-exposed plates, and ring-stage growth inhibition correlated positively with parasite clearance rates in all samples (r = 0.97, p<0.0001). P. falciparum isolates from this endemic area show sensitivity to artemisinin drugs both in-vivo and ex-vivo. The RSA method has potential to detect declines in parasite responses to artemisinins and can be used for continuous monitoring of responses especially in areas where resistance has not emerged.

Item Type: Conference or Workshop Item (Poster)
Subjects: R Medicine > RZ Other systems of medicine
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Dr. Olubanke Olujoke Ogunlana
Date Deposited: 06 Sep 2017 15:34
Last Modified: 06 Sep 2017 15:34

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