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Partial Purification and Kinetic Properties of Polygalacturonase from Solanum macrocarpum L. Fruit

Chinedu, S. N. and Dayo-Odukoya, Omolola Peace and Iheagwam, Franklyn N (2017) Partial Purification and Kinetic Properties of Polygalacturonase from Solanum macrocarpum L. Fruit. Biotechmology, 16 (1). pp. 27-33. ISSN 1682-296X

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Abstract

Background and Objective: Polygalacturonase [Poly (1, 4-"-D-galacturonide) glucanohydrolase, E.C 3.2.1.15] is a subclass of pectinase that hydrolyzes the glycosidic linkages between galacturonic acid residues in polygalacturonans. Solanum macrocarpum ripening prompts pectinase production. The objective of this study was to examine the kinetic properties of polygalacturonase from Solanum macrocarpum L. fruit. Methodology: The enzyme was partially purified by ammonium sulphate precipitation and gel filtration. Protein content, polygalacturonase activity and kinetic parameters were determined. Results: The protein content and polygalacturonase activity of the fruit juice extracts were 0.63±0.02 mg mLG1 and 45.96±6.31 U mgG1 protein, respectively. A 1.7 and 108.3 fold increase in enzyme activity was achieved by ammonium sulphate precipitation and gel filtration, respectively. The enzyme had a Vmax of 76.92 unit mgG1 protein and Km of 0.92 mg mLG1. The pH profile of the enzyme showed three activity peaks at 3.0, 5.5 and 7.0. The enzyme was most active at pH 3.0 and showed optimal activity at 30EC. Rapid release of product was observed within the first 20 min of enzyme incubation. The Zn2+, Ca2+, Mn2+, Pb2+, Fe2+, Cu2+ and EDTA exhibited inhibitory effect on polygalacturonase activity whereas Mg2+ had stimulatory effect on the enzyme. Conclusion: It was concluded that the fruit of Solanum macrocarpum is a rich source of polygalacturonase. The enzyme is favourably comparable with that of a fungi source and could be further exploited for commercial production of the enzyme

Item Type: Article
Uncontrolled Keywords: Polygalacturonase, Solanum macrocarpum , industrial application, partial purification, kinetic properties
Subjects: Q Science > QH Natural history > QH301 Biology
S Agriculture > S Agriculture (General)
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Franklyn N IHEAGWAM
Date Deposited: 06 Sep 2017 10:55
Last Modified: 06 Sep 2017 11:02
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/9155

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