Obembe, Olawole O. and Aworunse, Oluwadurotimi S. and Bello, Oluwakemi A. and Ani, Abosede O. (2017) Multiple Shoots Induction from Indigenous Nigerian Pumpkin (Cucurbita pepo L.). Annual Research & Review in Biology. ISSN 2347-565X
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Abstract
Aim: The aim of this study is to develop a protocol for in vitro regeneration of a Nigerian indigenous pumpkin (C. pepo L.) via seedling-derived cotelydon, cotyledonary node and hypocotyl explants. Study Design: A combination of 0.00, 1.00, 2.00 and 3.00 mgl-1 of 6-Benzylaminopurine (BAP) and 0.00 or 0.05 mgl-1 of 2,4-Dichlorophenoxy acetic acid (2,4-D) for each explant type were set up in three replicates making a total of 36 culture vessels in the entire experimental set up. Five explants were cultured per combination. Place and Duration of Study: The work was conducted in the Plant Tissue Culture Laboratory of the Department of Biological Sciences, College of Science and Technology, Covenant University, Ota, Ogun State, Nigeria, between January 2017 and July 2017. Methodology: Hypocotyl, cotyledonary node and cotyledon explants derived from 4-week old
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BAP; 2,4-D; cotyledonary node; cotyledon; hypocotyl |
| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QK Botany |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Mrs Hannah Akinwumi |
| Date Deposited: | 08 Sep 2017 09:49 |
| Last Modified: | 16 May 2018 08:30 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/9259 |
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