@article{eprints18258,
           title = {Fecal Carriage of Colibactin-Encoding Escherichia coli
Associated With Colorectal Cancer Among a Student
Populace},
          volume = {11},
          number = {4},
          author = {P. A. Akinduti and Ovbiosa O. Izevbigie and Omobolanle A. Akindut and Ezekiel O Enwose and Emmanuel O. Amoo},
       publisher = {Oxford University Press},
           pages = {106},
         journal = {Open Forum Infectious Diseases},
           month = {April},
            year = {2024},
        keywords = {antibiotics; colibactin; colorectal cancer; Escherichia coli; fecal.},
             url = {http://eprints.covenantuniversity.edu.ng/18258/},
        abstract = {Fecal carriage of the colibactin (clb) gene in Escherichia coli is described as a source that could promote carcinogenesis, progressing to
colorectal cancer. The present study investigated the demographic, dietary, and antibiotic consumption variables as correlates for
fecal carriage of clb+/E coli among the student populace. In a randomized cross-sectional survey, E coli (N = 136) from the fecal
samples of eligible students were characterized and evaluated for antibiotic resistance, {\ensuremath{\beta}}-lactamase (blm), biofilm, virulence factor
production, and strain tryptophan reverse mutagenic activity. The encoded clb+/E coli were analyzed for correlates with principal
component analysis. Of all the E coli strains, a low rate of 2 clb+/E coli (1.5\%) and higher rates of biofilm (13.2\%) and blm producers
(11.8\%) were recorded among the mutant strains as compared with the nonmutant types. All the clb+/E coli showed complete
resistance to amoxicillin, Augmentin (amoxicillin and clavulanate), gentamicin, and trimethoprim/sulfamethoxazole. The fecal
clb-encoded E coli (1.5\%) were not associated with demographic status, fiber-based food (odds ratio [OR], 1.03; 95\% CI, 56.74?138.7;
P = .213), alcohol (OR, 1.27; 95\% CI, 61.74?147.1; P = .221), antibiotic consumptions (OR, 1.11; 95\% CI, 61.29?145.3; P = .222), and
handwashing (OR, 1.17; 95\% CI, 60.19?145.5; P = .216). The hierarchical cluster of blm+/E coli revealed high-level resistance with a
multiantibiotic resistance index ?0.2 (P {\ensuremath{<}} .05). Only 12\% of all strains were tryptophan mutant/blm+, and 1.5\% of clb+/ECblm+ were
observed in fecal samples with a 452?base pair size. Trimethoprim/sulfamethoxazole and biofilm production positively regressed with
clb expression (P {\ensuremath{>}} .05). Principal component analysis score plot indicated an association of clb+/ECblm+ with dietary pattern, alcohol,
blm, and hemolysin production. The combined activity of blm and biofilm production in the gut microbiota could promote clb+/E
coli colonization, facilitating genotoxin production and possible colorectal cancer induction.}
}