%L eprints18263
%T Plant Microbiome Engineering:
Principles, Methods, and Current Trends
%B Biotechnological Approaches to Sustainable Development Goals
%I Springer, Cham
%R 10.1007/978-3-031-33370-5_17
%A Kesioluwa E. Orukotan
%A Nzubechi Elughi Gift
%A Bowofoluwa Sharon Abimbola
%A Abimbola David Akinyosoye
%A Eze Frank Ahuekwe
%A Olubukola Oziegbe
%P 251-267
%X Global demand for increased food output is predicted to rise by at least 60%
in 2050 as a result of climate change and human population development.
Plant microbiome engineering involves composition modification and host
plant phenotypic enhancement which often results to increased crop yield and
healthy agricultural products. As a new strategy to improve agricultural
productivity and human health, it is vital to improving global food output and
optimization. Impacts of plant microbiome engineering have been reported in
enhanced plant growth, nutrient use efficiency, abiotic stress tolerance, and
disease resistance in agricultural practices. Bottom-up or top-down
approaches using engineering tools like phage integrases, integrative and
conjugative elements (ICEs), chassis-independent recombinase-assisted
genome engineering (CRAGE), and host range broad host range (BHR)
genome engineering tools have been demonstrated in non-model
microorganisms. Emerging areas for microbiome engineering include soil
additives, artificial microbial consortia, microbiome breeding and
transplantation, and host-mediated microbiome. A common issue with the
engineering tools is overcoming restriction/modification of the target bacteria
because of their unique restriction/modification systems that defend them from
invading DNA, potentially limiting DNA transformation. Although most of the
engineering tools are effective for engineering bacteria, their throughput is
limited, as only a few hundred organisms can be designed at a time. To
reduce the limitations with the engineering tools, synthetic biology offers a
more reliable technique for microbiome engineering in situ to recode the
metagenome and generate desirable synthetic communities. These
techniques allow for the insertion and/or deletion of target activities in native
microbial communities with minimal disturbance to the context.
%D 2023