SHIGA, TOXIN-PRODUCING ESCHERICHIA COLI O157:H7 IN PACKAGED DRINKING-WATER IN ABEOKUTA, NIGERIA

Ajibola, Abiola and Shittu, Olufunke and Oluwasanya, Abidemi Ojo4 and Akinduti, P. A. and Akintokun, Aderonke (2023) SHIGA, TOXIN-PRODUCING ESCHERICHIA COLI O157:H7 IN PACKAGED DRINKING-WATER IN ABEOKUTA, NIGERIA. POLISH JOURNAL OF NATURAL SCIENCES, 38 (1). pp. 19-29.

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Abstract

There is a need to carry out regular microbiological analysis of packaged sachet water (PSW) sold in Nigeria to determine whether they comply with drinking-water quality standards. One hundred and fifty three PSW samples from fifty-one packaged sachet water facilities (PWMFs) in Abeokuta were collected from retail outlets between April – July, 2014. Samples were analyzed for Escherichia coli using standard culture media. Latex agglutination serological test; sequencing of 16S rRNA gene and sequence similarity analysis yielded E. coli O157:H7 from two PSW (1.32%) from one PWMF (1.96%). Both E. coli O157:H7 isolates were Shiga toxin (stx1) positive but stx2 negative. The two E. coli O157:H7 strains exhibited different resistance patterns to ten (10) antibiotics belonging to seven (7) different classes. Each E. coli O157:H7 strain showed resistance to more than two classes of antibiotics with MIC ≥ 8 μg/ml. This paper showed that the not all packaged sachet water analyzed in this study are fit for drinking, and can even be source of multidrug resistant and Shiga, toxin-producing E. coli.

Item Type:	Article
Uncontrolled Keywords:	drinking-water quality, Escherichia coli, molecular identification, packaged sachet water, Shiga toxin.
Subjects:	Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	ORIGHOEYEGHA
Date Deposited:	18 Jul 2024 16:20
Last Modified:	18 Jul 2024 16:20
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/18254

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