Balogun, Daniel Oluwatobiloba and Covenant University, Theses Masters (2024) RESISTANCE TO PYRETHROIDS IN MOSQUITOES IN ADO-ODO OTA, OGUN STATE. Masters thesis, Covenant University.
![[img]](http://eprints.covenantuniversity.edu.ng/style/images/fileicons/application_pdf.png) |
PDF
Download (216kB) |
Abstract
The spread of malaria can be controlled through long-lasting insecticidal nets (LLINs) and indoor residual spraying (IRS) using pyrethroids. However, the increasing resistance to pyrethroids poses a significant challenge to effective vector control in Africa. This study assessed mosquito species composition in Ado Odo Ota, Ogun state, and evaluated the resistance levels to pyrethroids. A 6- month longitudinal entomological survey was carried out using light trap and larval sampling. Mosquitoes were collected from various selected towns in Ado Odo Ota, and Anopheles species were identified through morphological and molecular analyses. The susceptibility status of Anopheles gambiae sensu lato to permethrin (0.75%) and deltamethrin (0.05%) was assessed using the World Health Organization insecticide susceptibility test. Additionally, the species were screened for knockdown (kdr) target site resistance alleles. Five hundred seventy-nine (579) adult mosquitoes were collected and morphologically identified using Coetzee’s taxonomic keys. Twothirds (376/579) of the mosquitoes were identified as Culex spp., constituting the most abundant species in Ado Ota local government. Molecular identification using intentional mismatch primers confirmed Anopheles coluzzi's presence as the communities' main species. Thirty percent 30% (22.5 – 37.5) of the Anopheles gambiae mosquitoes were susceptible to permethrin after 24 hours, while 98% (95.7 -100) of the vectors were susceptible to deltamethrin. Sporozoite detection using circumsporozoite ELISA showed none (0/40) of the tested blood-fed female Anopheles gambiae were positive for sporozoites, and the West African and East African knockdown resistance genes were detected in very high frequencies among the sampled population (L1014F – 64% [0.36 – 0.83], L1014S – 60% [0.30 – 0.77]. This study revealed that Anopheles coluzzi (99%) is the predominant species in Ado Odo Ota local government area. Deltamethrin is still effective in several communities; however, the high knockdown resistance of both the east and west alleles calls for urgent implementation of integrated vector control in Ogun state.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | Insecticide testing, Malaria, Mosquitoes, Pyrethroids, Species composition. |
| Subjects: | Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Patricia Nwokealisi |
| Date Deposited: | 30 Sep 2024 10:51 |
| Last Modified: | 30 Sep 2024 10:51 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/18467 |
Actions (login required)
 |
View Item |
