ALADELE, Andrew Kolawole and Covenant University, Theses Masters (2024) MOLECULAR DYNAMICS OF TUBER AND SEED YIELD IN Sphenostylis stenocarpa (Hochst. ex A. Rich. Harms) (AFRICAN YAM BEAN). Masters thesis, Covenant University.
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Abstract
Sphenostylis stenocarpa (African yam bean - AYB) is an understudied and opportunity crop with the potential to contribute to food security It is a versatile legume that produces both edible seeds and tubers. AYB faces under-exploitation due to limited understanding and challenges as well as an unabetted threat to its diversity like several other indigenous plant species. Expanding the diversity of global food sources has become imperative. This study investigated the molecular dynamics in AYB by assessing the genetic diversity in non-tuber and tuberproducing landraces, identified SNP markers associated with tuber formation and determined the relationship between seed and tuber production. The study evaluated accessions from the Genetic Resources Centre of the International Institute of Tropical Agriculture (IITA), Ibadan. Phenotypic data was generated from monitored growth, genotyping was conducted using DArTseq technology, with SNP data generated afterward. Phenotypic data employed clustering, correlation analysis and distribution plots. Genome-wide Association Studies (GWAS) using the GAPIT package in R elucidated population structure and identified SNPs responsible for yield. Multiple traits such as NoPods, Hundred sdcount, TSdwghtPPL, TPodwgth indicate a close association with each other showing a strong indication with the accessions and yield. While clustering for seed and tubers showed four and five clusters each. BLUEd traits and 2254 SNP markers from 92 genotypes were used for the association analysis. Using the BLINK, FarmCPU, GLM, and MLM models. Twelve significant SNP markers were identified to be associated with three African yam bean yield traits (Tuber weight, Seed thickness, and Pod width). These results have the potential to accelerate marker-assisted selection in molecular breeding.
| Item Type: | Thesis (Masters) |
|---|---|
| Uncontrolled Keywords: | African yam bean, opportunity, Seed, Tuber, SNPs, Yield |
| Subjects: | Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | nwokealisi |
| Date Deposited: | 07 Nov 2024 13:22 |
| Last Modified: | 07 Nov 2024 13:22 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/18570 |
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