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Production of yam mosaic virus monoclonal antibodies in mice peritoneum

Eni, A. O. and Hughes, J. d' A. and Rey, M. E. C (2011) Production of yam mosaic virus monoclonal antibodies in mice peritoneum. African Journal of Biotechnology, 10. pp. 11178-11181. ISSN 1684–5315

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Abstract

Yam mosaic virus (YMV) is one of the most economically important virus infecting yams. Immunoassays are routinely used for laboratory diagnosis of YMV and for certification of planting materials. However, YMV antibodies, the key reagents, needed for these immunoassays are not readily available. We describe in this paper, the production of YMV monoclonal antibodies for the detection of YMV. The monoclonal antibody was produced by immunizing six weeks old BALB/c mice with YMV hybridoma cells and tapping soft peritoneal tumor tissues for antibody. Antibody titre was determined by triple antibody sandwich-enzyme-linked immunosorbent assay (TAS-ELISA) using YMV infected yam leaves and non-infected tissue culture yam leaves. The antibody produced had a titre of 1:1,310,720 and an optimal TAS-ELISA detection dilution of 1:80,000. This high-titre YMV monoclonal antibody is useful for monitoring and certification purposes.

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 06 Feb 2014 08:46
Last Modified: 06 Feb 2014 08:46
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/2111

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