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Production of Polyclonal Antibody Against an Isolate of Yam-infecting Badnavirus from Nigeria

Eni, A. O. and Hughes, J. d' A. and Rey, M. E. C. (2010) Production of Polyclonal Antibody Against an Isolate of Yam-infecting Badnavirus from Nigeria. Australian Journal of Basic and Applied Sciences, 6 (5). pp. 4158-4163. ISSN 1991-8178

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Integrated viral sequences and high sequence variability among badnaviruses complicates the development of specific reliable molecular detection tests for yam-infecting badnaviruses. Thus Serological techniques are of notable importance for routine testing and monitoring of these viruses. The major limiting factor to the use of serological techniques is the limited availability of antibodies. Rabbit polyclonal antibody was produced against a purified preparation of a yam-infecting badnavirus from Nigeria. Antibody titre was determined by Protein-A sandwich (PAS) enzyme-linked immunosorbent assay (ELISA). The antibody produced had a titre of 1:1280 in PAS-ELISA and detected yam-infecting badnaviruses in infected yam leaves from Nigeria, Ghana, Benin and Togo. The suitability of the antibody for use in immunocapture polymerase chain reaction (IC-PCR) was evaluated. The antibody successfully trapped both Dioscorea alata bacilliform virus (DaBV) and Dioscorea sansibarensis bacilliform virus (DsBV) for IC-PCR detection. The antibody produced in this study will enhance certification of yam planting materials across West Africa and also facilitate the safe international movement of yam germplasm.

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 06 Feb 2014 08:47
Last Modified: 06 Feb 2014 08:47

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