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Assessment of Growth and Cellulase Production ofWild-Type Microfungi Isolated from Ota, Nigeria

Chinedu, S. N. and Eni, A. O. and Adeniyi, Adebayo I. and Ayangbemi, J. A. (2010) Assessment of Growth and Cellulase Production ofWild-Type Microfungi Isolated from Ota, Nigeria. Asian Journal of Plant Sciences, 9 (3). pp. 118-125. ISSN 1682-3974

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Abstract

The aim of the study was to isolate and identify filamentous microfnngi involved in wood-waste decomposition in Canaanland, Ota, South-West Nigeria and to evaluate their potentials for cellulose saccharification. Microbiological techniques were used to isolate and identify the ftmgi. Four filamentous microfnngi, identified as Aspergillus niger, Aspergillusflavus, Penicillium chrysogenum and Trichoderma sp., were isolated. All the isolates, particularly Trichoderma sp., grew rapidly on Sabouraud's agar and Czapek-Dox agar. Two of the isolates, Aspergillus niger and Trichoderma sp., was cultivated for 168 h by submerged fermentation in modified Czapek-Dox liquid medilllll containing cellulose as sole carbon source and harvested at 24 h intervals. The mycelia weight of the harvested cultures, and the protein content and cellulase activity of the filtrates were determined. The peak mycelia weight of 4.6 and 3.0 mg mL - 1 was, respectively obtained for Trichoderma sp. and A. niger at 48 h. The protein and cellulase activity of Trichoderma sp. peaked at 72 h whereas for A. niger, the peak protein content and peak cellulase activity was obtained at 96 and 72 h, respectively. The peak protein and cellulase activity values of A. niger were 0.175 and 0.077 nnit mL - 1 , respectively. Trichoderma sp. yielded a protein peak of 0.180 mg mL - 1 and peak cellulase activity of 0.108 nnit mL - 1 . There is a correlation between the protein content and cellulase activity of the culture filtrates. The strains of A. niger and Trichoderma sp. obtained from this study are potential tools for the saccharification and bioconversion of cellulosic materials

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 06 Feb 2014 08:47
Last Modified: 25 Nov 2015 13:55
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/2115

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