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Detection of Extracellular Enzymes in Microbial Isolates from diseased Carrot (Duacus Carota) Fruits

Ajayi, A. A. and Ubon-Isreal, Nsikakabasi Ntiense and Olasehinde, G. I (2011) Detection of Extracellular Enzymes in Microbial Isolates from diseased Carrot (Duacus Carota) Fruits. International Journal of Advanced Biotechnology and Research, 2. pp. 244-249. ISSN 0976-2612

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Abstract

A research work was carried out on diseased carrots obtained from the main Sango Ota market to isolate microorganisms associated with post harvest deterioration of carrots. Small portions (2 x 5mm) of the diseased parts of carrots were aseptically inoculated on Nutrient agar and Saboraud agar incubated at 37OC and 26OC respectively. The fungal species isolated were Aspergillus niger, Penicillium notatum, Mucor spp. and Fusarium spp. and they were identified using morphology and microscopy. The bacterial species isolated were Bacillus spp., Leuconostoc spp., Xanthomonas spp and Klebsiella spp and they were identified using microscopy, morphology and various Biochemical tests. Extracellular enzyme production was carried out on the isolates to test for their ability to elaborate Amylase and Protease. All the isolates except Aspergillus niger produced Amylase. Leuconostoc spp., Xanthomonas spp., Klebsiella spp. and Aspergillus niger elaborated Protease. The results of this present investigation with further studies on carrots can be utilized in carrot juice production which is not commonly available in Nigeria.

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 12 Mar 2014 09:23
Last Modified: 12 Mar 2014 09:23
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/2176

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