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Isolation, characterization and extracellular enzyme detection of microbial isolates from deteriorated apple (malus domestica) fruits

Ayanda, Opeyemi Isaac and Ajayi , A. A. and Olasehinde, G. I and Dare, O. T. (2013) Isolation, characterization and extracellular enzyme detection of microbial isolates from deteriorated apple (malus domestica) fruits. International Journal of Biological and Chemical Sciences, 7 (2). pp. 641-648. ISSN 1991-8631

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Abstract

Studies were carried out on deteriorated apples obtained from the Just Rite supermarket, Ota, Ogun State, Nigeria, to isolate microorganisms associated with post-harvest deterioration of apple (Malus domestica) fruits. The bacterial species were identified using microscopy, morphology and various biochemical tests while macroscopy and morphology was used to identify the fungi isolated. The ability of the isolate to elaborate extracellular amylase and protease were tested for. Results revealed Aspergillus niger and Aspergillus flavus as the fungal species while the bacterial species isolated were Bacillus spp and Micrococcus spp. All the isolates except Aspergillus niger produced amylase in substantial amount. Micrococcus spp, Aspergillus niger and Aspergillus flavus produced protease. The results of this investigation if combined with further studies can be used in identifying organisms which could be used as a biological method in the control of apple pathogens. © 2013 International Formulae Group. All rights reserved.

Item Type: Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mrs Patricia Nwokealisi
Date Deposited: 19 Mar 2014 13:31
Last Modified: 19 Mar 2014 13:31
URI: http://eprints.covenantuniversity.edu.ng/id/eprint/2183

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