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Genetic Diversity of Plasmodium falciparum Field Isolates from South Western Nigeria

Olasehinde, G. I and Yah, S. C. and Singh, R. and Ojuronbge, O Olusola and Ajayi, A. A. and Valecha, Neena and Abolaji, A. O. and Adeyeba, Adegboyega O. (2012) Genetic Diversity of Plasmodium falciparum Field Isolates from South Western Nigeria. African Health Sciences, 12 (3). pp. 355-361.

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Background: Plasmodium falciparum the main causative agent of malaria is an important public health vector. With the use of PCR, its genetic diversity has been extensively studied with dearth information from Nigeria. Methods: In this study, 100 P. falciparum strains merozoite surface protein 1( msp-1), merozoite surface protein 2 (msp-2) and Glutamate rich protein (Glurp) from Ogun State General Hospitals were characterized. The genetic diversity of P. falciparum isolates was analyzed by restriction fragment length polymorphism following gel electrophoresis of DNA products from nested polymerase chain reactions (PCR) of their respective allelic families KI, MAD 20, RO33 (MSP-1);FC27, 3D7 (MSP-2) and Glutamate rich protein respectively. Results: Majority of the patients showed monoclonal infections while multiplicity of the infection for msp-1 and msp-2 were 1.1 and 1.2 respectively. The estimated number of genotypes was 8 msp-1 (4 KI; 3 MAD; 1 RO33) and 6 msp-2 (3 FC27; 3 3D7). 80% of the isolates coded for Glurp with allelic size ranged between 700 and 900 bp. Conclusion: The allelic distributions however were similar to those previously reported in other endemic malaria countries. Future studies will be designed to include other malaria endemic regions of Nigeria such as the oil exploration regions

Item Type: Article
Uncontrolled Keywords: Genetic diversity; Plasmodium; Merozoite surface protein, monoclonal infection
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User: Mr Solomon Bayoko
Date Deposited: 17 Jan 2015 21:03
Last Modified: 17 Jan 2015 21:03

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