Extraction of lycopene with cell wall degrading enzymes from tomato (Lycopersicon esculentum Mill) fruits deteriorated by Aspergillus niger

Ajayi, A. A. and Atolagbe, O. M. and Olasehinde, G. I and Ayanda, O.O and Adejuwon, A. O. (2013) Extraction of lycopene with cell wall degrading enzymes from tomato (Lycopersicon esculentum Mill) fruits deteriorated by Aspergillus niger. Nature and Science, 11 (4). pp. 110-113. ISSN 1545-0740

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Abstract

Lycopene is the carotenoid which gives tomatoes and other red fruits their characteristic colour. It is one of the most powerful antioxidants and singlet oxygen quenching agents. It has been found to be of great medical importance with various anticancer effects and its ability to ameliorate several other medical conditions. Freshly ripe tomato fruits of the Roma vf variety and the Ibadan local variety were allowed to deteriorate after infection with a 96-hr-old culture of Aspergillus niger. Extraction of cell wall degrading enzymes produced during the deterioration process was carried out ten days after inoculation of the tomato fruits. The crude enzymes were precipitated using ammonium sulphate precipitation technique and employed in the extraction of lycopene from tomato peels. The yield of lycopene was 45.25mg/kg and 45.86mg/kg for enzymes extracted from the Roma vf and the Ibadan local varieties of tomato fruits respectively. This study established an improvement in lycopene extraction with crude preparation of cell wall degrading enzymes and compared the yield from the two enzymes obtained from the two most commonly available varieties of tomato fruits in Nigerian markets.

Item Type:	Article
Uncontrolled Keywords:	Lycopene; Aspergillus niger; Tomato peels; cell wall degrading enzymes
Subjects:	Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mrs Hannah Akinwumi
Date Deposited:	20 Feb 2015 10:35
Last Modified:	04 Mar 2016 09:31
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/3562

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