Onyegeme-Okerenta, B. M. and Chinedu, S. N. and Okafor, U. A. and Okochi, V. I.
Antibacterial Activity of Culture Extracts of Penicillium chrysogenum PCL501: Effects of Carbon Sources.
Online Journal of Health and Allied Sciences.
Penicillium chrysogenum PCL501 produced β-lactam antibiotics when fermented with different agro-wastes: cassava
shavings, corncob, sawdust and sugarcane pulp. In vitro antibacterial activity of the culture extracts was tested against four
clinical bacterial isolates, namely, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. All
the culture extracts and standard drug (commercial Benzyl Penicillin) inhibited the growth B. subtilis and E. coli; the potency
varied with carbon source. Antibacterial activity of extracts from cultures containing cassava shavings and sugarcane pulp
was comparable with that of the standard drug. The MIC against the susceptible organisms was 0.20mg/ml for the standard
drug and ranged from 0.40 to 1.50mg/ml for the culture extracts. Neither the culture extracts nor the standard drug
inhibited K. pneumoniae and P. aeruginosa; the bacterial strains produced β-lactamase enzymes. Cassava shavings and
sugarcane pulp are indicated as suitable cheap carbon sources for the production of antibiotics by Penicillium chrysogenum
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