In vitro activity of Bryophyllum pinnatum and Detarium microcarpum p lants against Mycobacterium tuberculosis and other bacteria

OLUGBUYIRO, J.A.O In vitro activity of Bryophyllum pinnatum and Detarium microcarpum p lants against Mycobacterium tuberculosis and other bacteria. Natural Products Research Bulletin, 1. pp. 1-7. ISSN 1478-6427

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Abstract

Two Nigerian medicinal plants Bryophyllum pinnatum and Detarium microcarpum, reputed in traditional medicine for the treatment of several ailments were selected for this investigation. The plant materials were macerated with methanol then partitioned into four fractions using solvents of different polarities. The solvent extracts were evaluated for activities against Mycobaterium tuberculosis (Mtb) and five other pathogens: Staphylococcus aureus, Salmonella typhi, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli. The anti Mycobaterium tuberculosis (Mtb) assay used proportion method while agar disc diffusion method was applied for other bacteria. Hexane fractions of both plants proved very active against the test strains showing significant potency at 25µg/ml. The methylene chloride fractions of the two plant agents were also very active against Mycobaterium tuberculosis in a dose (25µg/ml) dependent manner compared with controls. The results revealed that the most potent fraction against Mycobaterium tuberculosis (Mtb) was hexane portion followed by methylene chloride layer of the two species. Also, the ethyl acetate fraction of BP possessed good antibacterial activity against the other test micro-organisms at 5mg and 2.5 mg strengths, D. microcarpum fractions were inactive against the bacteria. This study, in support of folkloric uses, presents Bryophyllum pinnatum and Detarium microcarpum as promising natural product agents for generating anti-infective against Mycobaterium tuberculosis (Mtb).

Item Type:	Article
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology
Divisions:	Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User:	DR JOSEPH OLUGBUYIRO
Date Deposited:	17 Mar 2016 15:21
Last Modified:	17 Mar 2016 15:21
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/6483

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