OPTIMIZATION OF PECTINASE PRODUCTION BY ASPERGILLUS NIGER USING CENTRAL COMPOSITE DESIGN

Ajayi, A. A. and Salubi, A. E. and Lawal, B. and Onibokun, E. A. and Ajayi, M. O. and Ogunleye, T. A. (2018) OPTIMIZATION OF PECTINASE PRODUCTION BY ASPERGILLUS NIGER USING CENTRAL COMPOSITE DESIGN. AFRICAN JOURNAL OF CLINICAL AND EXPERIMENTAL MICROBIOLOGY, 19 (4). pp. 314-319. ISSN 1595-689X

PDF Download (283Kb)

Abstract

Pectinases are a group of enzymes that catalyze the breakdown of pectin. Pectinase producing Aspergillus niger was obtained from a five-day old Eba (Cassava flakes). Response surface methodology was used for optimizing the process of the pectinase produced. Four independent variables which are, temperature, pH, substrate concentration and time of Heating at 70oC were used to optimize the significant correlation between the effects of the variables on pectinase production. A second-order polynomial was fitted to data and validated by ANOVA. The results revealed maximum pectinase production at pH 6.0, 50oC Temperature, 0.02% substrate concentration and the enzyme lost all its activity within 7 min of heating at 70oC. The study revealed that optimization of pectinase through RSM could improve the enzymatic characteristics and yield of the enzyme. The models used were highly significant with a correlation coefficient (R2) of 0.901

Item Type:	Article
Uncontrolled Keywords:	Aspergillus niger; Response Surface Methodology; Central Composite Design
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mrs Patricia Nwokealisi
Date Deposited:	17 Dec 2018 10:02
Last Modified:	17 Dec 2018 10:02
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/12210

Actions (login required)

View Item