UMANE, Otibhor Phoebe and Covenant University, Theses (2018) A STUDY ON THE ANTIDIARHEIC POTENTIALS OF FERMENTED BAMBARA NUT (Vigna subterranea). Masters thesis, COVENANT UNIVERSITY.
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Abstract
In this study, nine isolates were obtained from a 96 h naturally fermented white Bambara nut. Comparative analysis of the proximate composition of fermented Bambara and raw samples were conducted. The parameters assessed include: protein, lipid, fibre, moisture,carbohydrate and ash contents. The predominant bacteria species that occurred during the fermentation period were isolated and purified and characterized using cultural, morphological and biochemical characterization and comparison with standard organisms. Molecular elucidations on the identity of the organisms were carried by gel electrophoresis and 16SrRNA characterization. The isolates were predominantly Bacillus species. Assay for the probiotic potential of the fermented Bambara was carried using selected Wistar albino rats. The rats were induced with diarrhea using clinical species 1011CFU/ml E. coli CFU/ml. Results obtained from the proximate analysis of the fermented nut include (%) carbohydrate 46.69; lipid 10.36; fibre 3.45, protein 15., ash content 3.3, moisture content 21.2 while for the raw sample (%) carbohydrate 37.37; lipid 7.68; fibre 2.95 protein 19; ash content 3.7 and moisture content 29.3. For the probiotic assay, using the histopathology assessment the diarrheic Wistar rat internal lumen showed necrosis which recovered following feeding of the fermented Bambara nut. This study showed that fermented Bambara, an underutilized legume could provide probiotics activities on consumers of such fermented product
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Mrs Hannah Akinwumi |
| Date Deposited: | 10 Mar 2020 11:47 |
| Last Modified: | 10 Mar 2020 11:47 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/13184 |
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