OKUNOLA, OLUWASEUN JOY and Covenant University, Theses (2018) MICROBIAL DIVERSITY OF SACHET WATER PACKS AND ITS HEALTH IMPLICATIONS. Masters thesis, COVENANT UNIVERSITY.
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Abstract
Safe drinking water is water with microbial, chemical and physical characteristics that meet WHO guidelines on drinking water quality. The aim of this study was to determine the microbial diversity of different sachet water packs and water marketed in Ota, Ogun State, Nigeria and assess their health implication. Sachet water, swabs of manufacturer’s hands, transport vehicle, water sachets and hands of hawkers and consumers were collected. Total aerobic plate count, coliform count and fungal count were carried out using standard microbial procedures. Isolates were subjected to morphological, biochemical and molecular characterization. Physicochemical analysis was also carried out on the water samples. Total aerobic plate count was in the range of 1×102 to 6.3×103 cfu/ml, coliform ranged between 1×102 – 2.1×103 cfu/ml for sachet water packs and hand swabs, while, total aerobic plate count of water samples ranged from 1×102 - 1.7×102 cfu/ml and that of coliform ranged from 1×102 - 6×101 cfu/ml. Predominant isolates identified includes; Shigella, Salmonella, Staphylococcus aureus, Escherichia coli, Proteus spp, Enterococcus spp Bacillus spp, and Pseudomonas spp. Morphological characterization of fungi revealed the presence of Aspergillus spp, Penicillium and yeast. The ressult of this investigation revealed that the sachet water brands sampled did not conform to Nigeria industrial standard and WHO standard. It is recommended that adequate treatment process should be utilized for production of quality and safe packaging materials and regulatory bodies should enforce strict hygienic measures in this rapidly expanding industry.
| Item Type: | Thesis (Masters) |
|---|---|
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Medicine, Health and Life Sciences > School of Biological Sciences |
| Depositing User: | Mrs Hannah Akinwumi |
| Date Deposited: | 30 Jun 2020 17:06 |
| Last Modified: | 30 Jun 2020 17:06 |
| URI: | http://eprints.covenantuniversity.edu.ng/id/eprint/13437 |
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