Nwachukwu, N and Iweala, E. E. J.
(2009)
INFLUENCE OF EXTRACTION METHODS ON THE HEPATOTOXICITY
OF AZADIRACHTA INDICA BARK EXTRACT ON ALBINO RATS.
Global Journal of Pure and Applied Sciences, 15 (3&4).
pp. 369-372.
ISSN 1118-057
Abstract
The influence of extraction methods: Cold aqueous (CA) hot aqueous (HA) and alcoholic extraction (AE)
methods on the hepatotoxic effect of Azadirachta indica bark extract (ABC) was investigated using albino rats. A total
of forty eight rats were divided into three groups of sixteen rats equally for the extraction methods. Each group was
subdivided into four rats to represent control, low, medium and high doses of the extract. The control group received
no extract while the other groups were administered the various concentrations (doses) intraperitoneally twice daily
for seven (7) days before they were all sacrificed under light chloroform and used for the analysis. Parameters
determined include glucose, cholesterol and triglycerol. Also alkaline phosphatase (ALP), Aspartate amino transferase
(AST) and alanine amino transferase (ALP) activities were determined. The result shows that glucose levels
decreased from 85.75+2.85 to 65.00+12.05 in high concentration of C.A. Similar decreases were obtained from HA
and AE extraction methods. Cholesterol levels also decreased from 111,63+1.02 to 53.75+0.90 in the high
concentration CA, and similarly decreased in HA and AE extraction methods. Also triglycerol levels decreased from
66.49+0.95 to 21.02+0.30 in the high concentration of CA, and also decreased respectively in both HA and AE
extraction methods. Similarly, the activities of the enzymes decreased in all the extraction methods, and for the
different concentrations over the control except for the activities of ALP which increased generally in CA method. This implies that ABE using HA and AE methods in non hepatotoxic while there is possibility of hepatotoxicity of the obstructive type if CA extraction method is used.
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