Prevention of Bacterial Biofilms Formation on Urinary Catheter by Selected Plant Extracts

Adesina, T. D. and Nwinyi, Obinna and OLUGBUYIRO, J.A.O (2015) Prevention of Bacterial Biofilms Formation on Urinary Catheter by Selected Plant Extracts. Pakistan Journal ofBiological Sciences, 8 (2). pp. 67-73. ISSN 1028-8880

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Abstract

In this study, we investigated the feasibility of using Psidium guajava, Mangifera indica and Ocimum gratissimum leaf extracts in preventing Escherichia colibiofilm fonnation. The plants extractions were done with methanol under cold extraction. The various concentrations 5.0, 10.0 and 20.0mg mL - 1 were used to coat 63 catheters under mild heat from water bath. Biofilm fonnation on the catheter was induced using cultures of E. coli. Biofilm fonnation was evaluated using aerobic plate count and turbidity at 600 nm. From the obtained results, Psidium guajava, Mangifera indica and Ocimum gratissimum delayed the onset of biofilm fonnation for a week. Ocimumgratissimum coated catheter had the highest inhibitory effect at 5.0, 10.0 and 20.0 mg mL - 1 with bacterial count ranging from 2.2xl05-7.0xl04 and 5.7xl05-3.7x105 for 120 and 128 h, respectively. The Psidium guajava coated catheter had the lowest inhibitory effect at 5.0, 10.0 and 20.0 mg mL-\ with bacterial count ranging between 4.3 xl05-1.9x103 and 7.7x105-3.8 xl05 for 120 and 128 h, respectively. Despite the antimicrobial activities, the differences in the activity of these plant extracts were statistically not significant (p<0.05)

Item Type:	Article
Uncontrolled Keywords:	Psidium guajava, Mangifera indica, Ocimum gratissimum, E. coli biofilm, catheters
Subjects:	Q Science > QD Chemistry
Divisions:	Faculty of Engineering, Science and Mathematics > School of Chemistry
Depositing User:	Mrs Patricia Nwokealisi
Date Deposited:	09 Oct 2015 10:44
Last Modified:	09 Oct 2015 10:44
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/5578

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