Investigation of Angiostensin-converting enzyme inhibitory potential and allergenicity of Sesamum indicum Linn seed proteins by an in silico approach (LB541)

Rotimi, Oluwakemi A and Oluwagbemi, Olugbenga and Iyanda-Joel, Wisdom and Omotosho, O. E. and Adebiyi, Marion O. and Adelani, Isaacson and Rotimi, S. O (2016) Investigation of Angiostensin-converting enzyme inhibitory potential and allergenicity of Sesamum indicum Linn seed proteins by an in silico approach (LB541). The FASEB journal. ISSN 0892-6638 • Online ISSN: 1530-6860

PDF Download (123kB)

Abstract

The nutraceutical role of dietary proteins and peptides is gaining considerable interest in the management of cardiovascular disease like hypertension. In order to investigate the contribution of Sesamum indicum seed proteins to its antihypertensive properties was investigated, there seed protein sequences: 7S globulin (NCBI accession number, giǀ13183177), 2S albumin (NCBI accession number, giǀ13183174) and 11S globulin (NCBI accession number, giǀ13183172) were selected for BLAST, BIOPEP and allergenicity analysis. BLAST gave the following similarities: 7S globulin vs rice glutelin precursor (35%), 7S globulin vs oat 12S seed storage globulin 1 (29%), 11S globulin vs rice glutelin precursor (41%), 11S globulin vs oat 12S seed storage globulin 1 (39%) and 2S albumin vs oat 12S seed storage globulin 1 (60%). BIOPEP analysis showed that the sesame proteins demonstrated either di- or tri-peptide with a total of 92, 91 and 34 potential ACE inhibitory peptides from 7S globulin, 2S albumin and 11S globulin, respectively. Papain hydrolysis theoretically released the highest numbers of predicted ACE inhibitory peptides (23, 14 and 5) from 7S globulin, 2S albumin and 11S globulin, respectively. The 8-mer and 80-mer allergenicity analysis of these proteins showed significant matches with allergenic proteins from Lens culinaris, Anacardium occidentale, Carya illinoinensis. Although, the ccombined digestion with pepsin, trypsin and chymotrypsin A, a simulation of human gastrointestinal digestion, gave a sum of 32 predicted ACE inhibitory peptides from these proteins, their allergenic property may be a limitation

Item Type:	Article
Subjects:	Q Science > QA Mathematics > QA75 Electronic computers. Computer science Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Engineering, Science and Mathematics > School of Electronics and Computer Science
Depositing User:	Mrs Patricia Nwokealisi
Date Deposited:	02 Mar 2017 13:45
Last Modified:	02 Mar 2017 13:45
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/7866

Actions (login required)

View Item