Oxidative stress biomarkers in Clarias gariepinus (Burchel, 1822) exposed to Microcystin-LR

Isibor, Patrick Omoregie (2017) Oxidative stress biomarkers in Clarias gariepinus (Burchel, 1822) exposed to Microcystin-LR. Beni-Suef University Journal of Basic and Applied Sciences. pp. 69-75.

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Abstract

Stress levels inflicted by microcystin-LR extracted from Microcystis aeruginosa were assessed by estimating the effects on the oxidative stress biomarkers in the liver, muscle and gill tissues of Clarias gariepinus. Microcystin-LR was administered to the fish by injection into their intra-peritoneal cavity. Microcystin- LR induced the activities of mainly Glutathione-S-Transferase (GST), Catalase (CAT) and Superoxide dismutase (SOD) in the liver on dose and temporal basis, while it inhibited the activities of Cytochrome P450 on temporal basis in the liver and gill. Results show that MC-LR significantly induced oxidative stress in the C. gariepinus. Findings show that 400 lg MC-LR/kg b.w. disrupted the antioxidant status of the fish without any external physical manifestation on the body. Microcystin-LR induced the activities of mainly GST, CAT and SOD in the liver on dose and temporal basis while it inhibited the activities of CYP450 on temporal basis in the liver and gill. The adopted MC-LR doses; particularly 400 lgMC-LR/kg b.w. inflicted stress on the fish without any external/physical manifestation. MC-LR impacted on the antioxidant enzymes in the fish; in the order of liver > gill > muscle. This research has provided empirical evidences that 400 lg MC-LR/kg b.w induced oxidative stress in C. gariepinus in less than a month of exposure. This adopted level of MC-LR can be used as a warning signal in natural water bodies and commercial C. gariepinus ponds.

Item Type:	Article
Uncontrolled Keywords:	Microcystin-LR Microcystis aeruginosa Oxidative stress Hepatotoxin Clarias gariepinus
Subjects:	Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology
Divisions:	Faculty of Medicine, Health and Life Sciences > School of Biological Sciences
Depositing User:	Mrs Patricia Nwokealisi
Date Deposited:	22 Nov 2019 11:45
Last Modified:	22 Nov 2019 11:45
URI:	http://eprints.covenantuniversity.edu.ng/id/eprint/12976

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